Fig. 1

Genomic profiles of bulk tumor samples. a Multiregional sampling scheme. A total of 18 samples were collected, including two samples from healthy tissue (in blue), eight from the primary tumor (green), two from proximal colonic lymph nodes (gold), two from distal hepatic lymph nodes (salmon), and four from liver metastasis (red). Scale bar: 1 cm. b Principal component analysis (PCA) with variant allele frequencies (VAF) for all 475 somatic mutations detected. Each circle corresponds to a given sample, with colors highlighting the anatomical regions. c Heatmap depicting genome-wide allele-specific copy-number status (from 0 in blue to 4 in red) of healthy and tumor samples. Sample IDs are shown at the top. d Heatmap with the observed allele frequencies (from 0 in white to 0.65 in red) of somatic mutations identified in the sequenced samples. Here only the non-synonymous mutations are shown (n = 156), sorted according to their mean VAF across all tumor samples. Gene names are displayed at the bottom of the map. Each row represents a single sample