Fig. 3
From: Rewiring carbon metabolism in yeast for high level production of aromatic chemicals
Validation of a phosphoketolase-mediated E4P generation route in the basic PAL branch strains. a Schematic overview of phosphoketolase (PHK)-based pathway for the generation of E4P for p-HCA production. The heterologous PHK pathway, consisting of a phosphoketolase from Bifidobacterium breve (BbXfpk) and a phosphotransacetylase from Clostridium kluyveri (CkPta), was introduced into the PAL branch-based (including a phenylalanine ammonia lyase and a cinnamic acid hydroxylase) strain. Ace acetate; see Fig. 1 legend regarding abbreviations of other metabolites. b Introduction of the PHK pathway in combination with feedback-insensitive DAHP synthase (ARO4K229L), and chorismate mutase (ARO7G141S) increases p-HCA titers via the PAL branch. c The PHK pathway outperforms the native E4P-generating route via Tkl1 for p-HCA production. d Combining the PHK pathway with deletion of the native glycerol-1-phosphatase-encoding gene GPP1 (marked with a red cross in a) enhances p-HCA production. Cells were grown in defined minimal medium with six tablets of FeedBeads as the sole carbon source, and cultures were sampled after 96 h of growth for p-HCA detection. Statistical analysis was performed by using Student’s t test (one-tailed; two-sample unequal variance; *p < 0.05, **p < 0.01, ***p < 0.001). All data represent the mean of n = 3 biologically independent samples and error bars show standard deviation. The source data underlying figures b–d are provided in a Source Data file
