Fig. 2

Loss of CD47 in IEC does not induce immune-mediated mucosal damage. a–c Naive Cd47^ΔIEC and tamoxifen-treated Cd47^ERΔIEC mice housed in pathogen-free conditions were analyzed for intestinal epithelial CD47 expression. Cd47^ERΔIEC mice were treated with tamoxifen to induce acute CD47 deletion in intestinal epithelial cells, and analyzed 2 weeks later. a Tissue sections from naive Cd47^ΔIEC and tamoxifen-treated Cd47^ERΔIEC mice were stained with anti-CD47 antibodies (green) with DAPI counterstain (blue). CD47 expression is absent in the epithelium but retained in the lamina propria and submucosa. Scale bars = 100 μm upper panels, 50 μm lower panels. b IECs  were isolated from the terminal ileum of naive mice. Protein lysates were analyzed by SDS–PAGE and immunoblot for CD47. c IECs were isolated from Cd47^ERΔIEC mice treated with vehicle (corn oil) or tamoxifen, and analyzed as in b. Results are representative of three independent experiments with 3–5 mice per group. d Paraffin-embedded colon tissue sections from Cd47^ΔIEC mice were stained with Hematoxylin and Eosin counterstain for histological examination. Gross mucosal architecture is intact in the absence of epithelial CD47 expression. Scale bars = 50 μm upper panels, 100 μm lower panels. e Colon tissue digests from naive Cd47^ΔIEC mice were stained and analyzed by flow cytometry, showing no significant differences in major lamina propria immune cell populations. Points represent individual samples each containing two mice (n = 4 mice per group). Data are means ± SEM and are representative of three independent experiments. Source data are provided as a Source Data file