Fig. 6
From: The long noncoding RNA lncNB1 promotes tumorigenesis by interacting with ribosomal protein RPL35
LncNB1 is required for neuroblastoma cell proliferation and survival. a BE(2)-C, Kelly, and CHP134 cells were transfected with control siRNA, lncNB1 siRNA-1, lncNB1 siRNA-2 for 96 h. Relative numbers of viable cells were examined by Alamar blue assays and expressed as percentage changes. b BE(2)-C and CHP134 cells were transfected with control siRNA, lncNB1 siRNA-1, or lncNB1 siRNA-2 for 96 h, followed by staining with Annexin V and flow cytometry analysis of Annexin V positively stained cells. c–e BE(2)-C, CHIP134, and/or Kelly cells were transfected with control siRNA, DEPDC1B siRNA-1, or siRNA-2 (c), RPL35 siRNA-1 or siRNA-2 (d), or E2F1 siRNA-1 or siRNA-2 (e) for 96 h. Relative numbers of viable cells were examined by Alamar blue assays and expressed as percentage changes. f DOX-inducible control shRNA, lncNB1 shRNA-1 or lncNB1 shRNA-2 BE(2)-C and Kelly cells were treated with vehicle control or DOX for 96 h, followed by staining with propidium iodide and flow cytometry analysis of the cell cycle. The percentage of cells at the S phase was shown. g DOX-inducible control shRNA, lncNB1 shRNA-1 or lncNB1 shRNA-2 BE(2)-C and Kelly cells were treated with vehicle control or DOX for 96 h, followed by staining with Annexin V and flow cytometry analysis of Annexin V-positive cells. Throughout, data were shown as the mean ± standard error of three independent experiments, and evaluated by two-sided unpaired Student’s t-test for two groups or one-way ANOVA for more than two groups. *, **, and *** indicate P < 0.05, 0.01, and 0.001, respectively. Source data are provided as a Source Data file
