Fig. 7
From: The long noncoding RNA lncNB1 promotes tumorigenesis by interacting with ribosomal protein RPL35
LncNB1 knockdown leads to tumor regression in neuroblastoma-bearing mice. a, b DOX-inducible control shRNA, lncNB1 shRNA-1 or shRNA-2 BE(2)-C and Kelly cells were treated with vehicle control or DOX for 14 [BE(2)-C cells] or 18 (Kelly cells) days. Cell colonies were fixed with crystal violet (a) and the numbers of colonies counted (b). Data were shown as the mean ± standard error of three independent experiments, and evaluated by two-tailed unpaired Student’s t-test. ***P < 0.001. c, d DOX-inducible lncNB1 shRNA-1 BE(2)-C cells were xenografted into nude mice. When the tumor reached 0.05 cm3, the mice were divided into two groups and given feed with or without DOX. Tumor growth was monitored, and the mice were culled when tumor size reached 1.0 cm3 or 6 months later. The effect of DOX on tumor progression was evaluated with two-way ANOVA (c). The survival curve showed the probability of overall survival of the mice, and P value was obtained from two-sided log-rank test (d). Source data are provided as a Source Data file
