Fig. 5
From: Using slow frame rate imaging to extract fast receptive fields
Voxel-timing glutamate filters from Drosophila visual neurons. a Image of Mi1 dendritic arbors expressing iGluSnFR. Five regions of interest (ROIs) corresponding to different cells are outlined with different colors. b A stochastic light intensity stimulus was presented to the fly, and updated at 60 Hz (top). The y-position of the scan oscillates at the frame rate (~13 Hz) and ROI fluorescence is captured at intervals shown with colored circles (middle). Colored dots indicate the fluorescence and capture time for the five ROIs (bottom). c A best-fit filter (receptive field) for a sample ROI (thick blue line in a) was extracted from the 13 Hz data upsampled to 60 Hz through linear interpolation (top). This filter is limited by the timescale of the 13 Hz sampling interval (black bar). Best fit filters were also extracted from the same underlying data using the voxel-timing method in conjunction with OLS and ASD (bottom). Error bars throughout are ±1 SEM confidence intervals computed by bootstrapping response samples. d Best fit filters as in c, but using a subsampling of the original data in order to simulate a 2.2 Hz acquisition. Filters were extracted from an interpolated response (top) or using the voxel-timing method (bottom)
