Fig. 2
From: Modulating multi-functional ERK complexes by covalent targeting of a recruitment site in vivo
BI-78D3 reaction with free thiols afford a stable tetrahedral intermediate. a, b Observed change in the UV-visible spectrum for the reaction of a 2-methoxyethanethiol (100 µM) or b ERK2 (50 µM) with BI-78D3 (10 µM) in 50 mM phosphate buffer, pH 7.5 and 2% dioxane (spectra recorded every 10 s for 1000 s). c Deconvoluted mass spectra of ERK2 showing the addition of approximately 386 Da after incubation of ERK2 (5 µM) with BI-78D3 (100 µM) for 15–20 min, followed by buffer exchange using a PD-10 column (calculated molecular weights of BI-78D3 and ERK2 are 380 and 42,329 Da respectively). d Proposed mechanism of the reaction between BI-78D3 and ERK1/2
