Fig. 7
From: Synergized regulation of NK cell education by NKG2A and specific Ly49 family members
The combined deficiency of inhibitory Ly49-family receptors and NKG2A severely impairs NK-cell education. a Poly(I:C)-primed splenocytes from the indicated mice were stimulated with tumour target cells. The percentages of IFN-γ+ or CD107a+ gated CD3−NKp46+ NK cells were analysed. b Poly(I:C)-primed splenocytes from indicated mice were stimulated by YAC-1 tumour targets. Frequency of IFN-γ+ of four NK-cell subsets (gated CD3−NKp46+), including DN (CD27−CD11b−), CD27 SP (CD27+CD11b−), DP (CD27+CD11b+) and CD11b SP (CD27−CD11b+) were analysed. c Similar to (a). d Similar to (b). e In vivo rejection of β2M-deficient splenocytes (similar to Fig. 3a). f In vivo rejection of β2M-deficient splenocytes (similar to Fig. 3d). Each symbol represents an individual mouse. Data shown represent two (e, f) or at least three (a–d) independent experiments. Mean ± SD is shown. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Unpaired Student’s t-tests (two-tailed) was used to calculate these values. Source data are provided as a Source Data file
