Fig. 1
From: Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability
Chaserr is essential for postnatal viability. a Genomic locus of Chaserr in the indicated vertebrate genomes. CAGE read coverage is from the FANTOM5 project75. 3P-seq data are from ref. 76. RNA-seq data are from HPA77, ENCODE (mouse), FAANG (pig), SRP009831 (lizard), SRP039546 (xenopus), and SRP024369 (zebrafish). b Left: single-molecule FISH with probes targeting Chaserr introns (red) and exons (green) in mouse embryonic fibroblasts. Chaserr exons are marked by white arrowhead. Scale bar shows 10 µm. Right: qRT-PCR comparing cytoplasm and nuclear fractions in mESCs. n = 5. c Left: Chaserr and Chd2 RNA expression across different tissues profiled in the mouse BodyMap78. Right: scatter plots for Chaserr and Chd2 expression in mouse BodyMap and human HPA77 projects. d Schematic of the positions of regions targeted by gRNAs used to generate Chaserr−/− and Chd2m/m mice. e Left: neonate survival rates for the indicated crossing. Right: embryonic survival rates at the indicated embryonic time points. f Weights of neonates at the indicated age (males n = 7–15, females n = 6–18 per group). Error bars show S.E.M. *P < 0.05, **P < 0.01, and ***P < 0.001 (e chi-squared test. f two-sided t test). Source data are provided as a Source Data file
