Fig. 5
From: Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy
RAP1 mediates interaction between XRCC4/Lig IV and DNA-PK. a Flag-tagged RAP1 mutants (as indicated) were expressed in cells followed by IP using anti-Flag beads. The IP eluate was immunoblotted for the indicated proteins. Inputs are shown in bottom panel. b MCF7 cells were transfected with shcontrol or shRAP1, and left untreated (UT) or treated with CPT (10 μM) for 3 h (represented as 0 time point). Following this, cells were released for various time points and fractionated and blotted for indicated proteins. WCE indicated whole-cell extract c Co-immunoprecipitation of lysates from shRAP1 or shControl cells UT or treated with CPT (10 μM) for 3 h and released for the indicated times. Immunoblots demonstrate association of indicated proteins.
