Fig. 7

RAP1 levels correlate with response to chemotherapy and patient survival. a IHC staining of RAP1 in breast cancer patient tissues. Staining intensity was classified into four groups: 0 (negative), 1 (weak), 2 (moderate), and 3 (strong). Staining scores 0 and 1 were grouped as negative immunoreactivity whereas 2 and 3 were grouped as positive immunoreactivity. Scale bar, 50 μm. b Graphs showing correlation of progression free and overall survival with RAP1 levels (n = 31). c, d For survival data analysis of colorectal cancers, raw gene expression data with GEO accession numbers of GSE17538 (n = 232) and GSE39582 (n = 1193) were downloaded from the GEO database (http://www.ncbi.nlm.nih.gov/geo/). Normalization of the raw data was performed across all samples based on the Cross Correlation method⁶³. Stratification of samples replied on the distribution of RAP1 (TERF2IP) expression intensities cross all samples. Ten percent below or over the RAP1 median expression value was used as the cutoff for selection of RAP1 lower or higher samples, respectively. The analysis of the survival data was based on the Kaplan–Meier method. e Model showing that RAP1 binds DDR complex proteins and regulates their formation and recruitment on chromatin.