Fig. 8
Conditional secretion of IL-12P70 increases antitumor activity of TRACCAR T cells in vivo. a Schematic showing the experimental design to investigate the antitumor activity of engineered TRACCAR T cells in xenograft mice model. Immunodeficient NSG mice were adoptively transferred on day 0 with RAJI-Luc-GFP tumor cells (2.5 × 105 cells per animal in 100 μL of PBS i.v.). Tumor cells were allowed to expand until mice randomization, performed at day 3 on the basis of the level of tumor cell bioluminescence signal. On the same day, mice were adoptively transferred (i.v.) with 7 × 106 viable mock-transduced T cells, TRACCAR T cells (n = 8), TRACCAR_ΔPD1 (n = 8), TRACCAR_ΔPD1IL12 (n = 7) T cells or tumor alone (n = 4). RAJI-Luc-GFP tumor cell expansion was monitored on various days by bioluminescence imaging (BLI) up to 142 days. b Evolution of average radiance as a function of time for the different mouse cohorts. Dotted lines correspond to the average radiance recorded for individual mouse. Comparison of tumor growth was done using the aucVardiTest function of the clinfun R package. P-value are indicated in the Supplementary Table 2. c Kaplan Meier plot obtained for different mice cohorts. Log-rank (Mantel-Cox) test was used for statistical analysis (p-value are indicated on the figures). Source data are provided as a Source Data file
