Fig. 4
Sustained beating-heart time-lapse imaging of immune cell responses to cardiac injury. a, b Sequence of MIPs (from Supplementary Videos 6–8) showing macrophages (a magenta mpeg1:mCherry) and neutrophils (b cyan mpx:mCherry) migrating to, and interacting at, the laser wound site (white circle) on the ventricle (myl7:GFP, laser injury has bleached myocardial cells at the injury site). Arrowheads mark tracked immune cells interacting with the wound margins, changing shape and reverse migrating from the wound to the pericardium. c, d Orthogonal plane views co-localising the tracked macrophages (c) and neutrophils (d) on and within the myocardium at the wound site. e, f Individual macrophages are seen to undergo complex shape changes during migration. All timestamps show hours post injury (hpi). Scale bars: 30 μm (10 μm for e, f)
