Fig. 5
Lgr5+ cells can initiate tumors with markers of HGSOC. a Schematic depicting crosses of Tp53R172H/−;T121;Lgr5Cre; and Rosa26-tdTomato mice. b Lineage tracing scheme for Lgr5Cre;Rosa26-Tdtomato mice. c Whole mounts of ovaries from LPT;Rosa26-Tdtomato mice induced with 4-OHT at 6 weeks (pulse), followed by sacrifice and analysis at the indicated times (chase), showing Tomato (red), and DAPI (blue) fluorescence; region within the white box in left top panel is magnified below. Note expansion of isolated Tomato+ cells into Tomato+ clones. Scale bar, 50 μm. d Corresponding H&E-stained sections from ovaries in c; e Relative areas of Tomato+ OSE clones at the indicated times after 4-OHT induction (chase times); clone size was determined by measuring the longest length of a discrete Tomato+ clone. Each circle represents a distinct Tomato+ clone, with n = 3 ovaries analyzed/time point, and a total of 180 clones counted (full data are provided as a Source Data file). f Quantification of hyperplastic clone size on the ovarian surface, determined by measuring the longest ‘length’ of epithelial protrusions (tumor cells) from the border with stromal cells. For each time point, 20 cross-sections from three ovaries were counted. Data represent mean ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001, Tukey’s multiple comparison test; Source data are provided as a Source Data file. g Representative IHC (Ki67 and Stathmin-1) and immunofluorescence (WT-1) staining for HGSOC markers in OSE from LPT mice at the indicated times after 4-OHT induction or without treatment; scale bars: 100 μm.
