Fig. 2

γ and β-actin isoforms differentially localize to cPM and pPM. a 3D-Airyscan images of a DC stained for γ (magenta) and β-actin (green). Insets depict a single podosome. b Average ± s.d. radial fluorescent intensity profile of γ and β-actin (n = 145 podosomes pooled from two independent experiments) at two different focal planes (z: 185 nm and z: 555 nm). c Quantification of the FWHM of the γ-actin fluorescent profile (n = 145 podosomes pooled from two independent experiments). d Quantification of the γ-actin ring diameter (n = 145 podosomes pooled from two independent experiments). e Dual-color STORM images of a DC stained for γ (magenta) and β-actin (green). Insets depict a single podosome. f Average radial orthogonal view of γ (magenta) and β-actin (green) acquired by STORM super-resolution. Bottom panel shows the merged images. g Schematic representation of the cPM and pPM in the podosome core. Scale bars: a = 2 µm, e = 1 µm, f = 0.5 µm; insets: a = 0.5 µm, e = 0.25 µm. FI fluorescent intensity, AU arbitrary units. Box plots indicate median (middle line), 25th, 75th percentile (box) and 5th and 95th percentile (whiskers) as well as outliers (single points). Source data are provided as a Source Data file