Fig. 5
OPG-Fas interaction mediates the OPG-induced phenotypic response of PASMC. TaqMan expression of (a) VEGFA, (b) PDGFRA, (c) TNC, (d) Cav1 and (e) TRAIL in response to OPG in the presence (hash bars) or absence (Grey bars) of anti-Fas neutralising antibody (1500 ng ml−1). Panel (f) demonstrates OPG inhibition of FasL and TRAIL-induced apoptosis in HT1080 cells. g PASMC migration following 6 h stimulation with PDGF (20 ng ml−1), OPG (30 ng ml−1) or 0.2% FCS (serum-free media, SFM), in the presence or absence of Fas neutralising antibody. h Proliferation of PASMCs following stimulation with OPG for 72 h in the presence or absence of Fas neutralising antibody and/or TRAIL neutralising antibody (0.5 nM). Proliferation expressed as a percentage of proliferation to PDGF. Bars represent the mean with error bars showing the standard error of the mean. Dots represent experimental repeats, Panels (a–e) (n = 4), panel (f) (n = 3), panel (g) (n = 4), panel (h) (n = 4 for SFM, 10 for PDGF & OPG stimulations) * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 following one-way ANOVA with Bonferroni’s multiple comparisons post hoc test
