Fig. 1
D2 and D3 are dynamically expressed during SCC tumor initiation and progression. a Schematic representation of the two-step carcinogenesis experiment. b Representative H&E, D2/D3 co-staining, and K8 and K14 staining of DMBA-TPA-treated skins for the indicated weeks. Images represent tumors of one of eight different D2-Flag mice analyzed for each time point (n = 8). Scale bar represents 200 μm. c mRNA levels of D2 and D3 were measured in progressive DMBA-TPA-treated skins as indicated in a and b, by real-time PCR analysis (n = 8). d Intracellular T3 was measured as indicated in the “Methods” section in dorsal skin tissues from mice treated with DMBA-TPA for 15 (PAP) and 30 (SCC). e Western blot analysis of D2-Flag and D3 expression from skin lesions of D2-3xFlag mice treated with DMBA-TPA for the indicated times (n = 8). HEK293 cells transfected with D2-Flag or D3 served as D2 and D3 controls, respectively. Arrows indicate the papillomas (PAP) and SCC tumors. f Western blot analysis of E-cadherin, vimentin, and tubulin in the same samples as in e. Quantification of the single protein levels versus tubulin levels and the E-cadherin/vimentin ratio is represented by histograms.
