Fig. 2
Increased thyroid hormone action in epidermal-specific D3KO mice enhanced the migration and invasion potential of SCC. a Schematic representation of D3-depletion and the two-step carcinogenesis experiment in 12 CTR and 12 sD3KO samples (n = 12). b Representative H&E and K6 staining of DMBA-TPA-treated CTR (n = 8) and sD3KO (n = 8) skins for 8 weeks. Scale bar represents 200 μm (top). mRNA levels of K6 and K14 were measured by real-time PCR analysis in 16 lesions from CTR mice and 16 lesions from sD3KO mice (bottom). c The number of skin lesions was counted during the two-step carcinogenesis experiment as indicated in a (ii). Tumor incidence is expressed as the number of tumors per mouse. d Representative images of the dorsal skin from CTR and sD3KO mice treated with DMBA-TPA for 15 weeks (n = 12). H&E of the skin lesions from CTR (n = 6) and sD3KO (n = 6) mice treated with DMBA-TPA for 15 weeks. Scale bar represents 200 μm. e mRNA levels of K8 in the dorsal skin of CTR (n = 15) and sD3KO (n = 15) mice. f mRNA levels of E-cadherin/N-cadherin in skin lesions of CTR (n = 15) and sD3KO (n = 15) mice measured by real-time PCR analysis. g Western blot analysis of E-cadherin, N-cadherin, and vimentin expression in skin lesions of CTR (n = 10) and sD3KO mice (n = 10). Quantification of the single protein levels versus tubulin levels and the E-cadherin/N-cadherin ratio is represented by histograms. *P < 0.05, **P < 0.01. h Schematic representation of the effects of D3-depletion on SCC tumor growth and progression.
