Fig. 1
From: Brain activity regulates loose coupling between mitochondrial and cytosolic Ca2+ transients
Mitochondrial morphology in the mouse M1 pyramidal neurons. Data were obtained with automated tape-collecting ultramicrotome scanning electron microscopy (ATUM-SEM). a Gallery view of SEM slices of the dendritic mitochondria. Yellow and green arrows indicate the mitochondria nanotunneling in the dendrite highlighted in pink. Scale bar: 1 μm. b Volume reconstruction of mitochondria (blue) in the dendrite (semi-transparent white) as shown by the pink area in (a). Yellow and green arrows indicate the mitochondria nanotunneling. c Image of a cell body of pyramidal neuron in M1. Scale bar: 5 μm. d 3D volume reconstruction of mitochondria in the mouse M1 neuron soma (semi-transparent white). n = 291 mitochondria identified are shown in different colors. Scale bar: 5 μm. e Statistics of mitochondrial length in dendrites and somas. n = 35 dendritic and 2465 somatic mitochondria. f Volume densities of mitochondria in dendrites and somas. n = 6 dendrites and 5 somas. Data are presented as mean ± SEM. *P < 0.05. **P < 0.01. ***P < 0.0001, unpaired T-test. Source data are provided as a Source Data file.
