Fig. 2

Banf1 binds to PARP1 following oxidative stress. a, b Banf1 and PARP1 are in a complex; immunoprecipitations from HEK293T cells expressing Flag, Flag-Banf1 or Flag-PARP1 using Flag antibodies. Immunoprecipitates were immunoblotted with the indicated antibodies. c The Banf1:PARP1 interaction after H₂O₂; immunoprecipitations from HEK293T cells ectopically expressing Flag or Flag-Banf1 1 h after H₂O₂ removal. Immunoprecipitates were immunoblotted with the indicated antibodies. d Banf1 and PARP1 directly interact. The indicated purified proteins (±NAD⁺/DNA/olaparib) were incubated together before immunoprecipitation with PARP1 antibodies and immunoblotting with the indicated antibodies. e Banf1 and PARP colocalise following oxidative stress induced by H₂O₂ in U2OS cells. Representative cells fixed at the indicated times following removal of H₂O₂ and stained with the indicated antibodies are shown, the colocalisation was analysed using ImageJ. The scale bar represents 10 μm. Immunoblots are representative of three independent experiments. Source data are provided as a Source Data file.