Fig. 3

Banf1 regulates PARP1 activity and repair of oxidative DNA damage. a Auto-poly-ADP-ribosylation of PARP1 in U2OS cells depleted of Banf1 following H₂O₂. The PAR bands were analysed via densitometry and normalised to γ-Tubulin. Two-way ANOVA was used for statistical analysis. b Auto-poly-ADP-ribose of PARP1 in U2OS expressing ectopic Flag-Banf1 following H₂O₂. The PAR bands were analysed via densitometry and normalised to γ-Tubulin. Two-way ANOVA was used for statistical analysis. c Inhibition of poly-ADP-ribose activity of PARP1 purified from HEK293T cells expressing ectopic Flag-Banf1 on an immobilised histone substrate following H₂O₂. Data shown represent the mean and S.D. of two independent experiments. Paired t-test was used for statistical analysis. d In vitro inhibition of PARP1 poly-ADP-ribose activity on histones by purified Banf1. e Alkaline comet assay showing the relative olive tail moment in Banf1-deficient and control U2OS cells. f Banf1 inhibits repair of oxidative DNA damage. Alkaline comet assay showing the relative olive tail moment in U2OS control cells and cells ectopically expressing Flag-Banf1. Paired t test was used for statistical analysis. Histogram data shown in f represent the mean and S.D. of four independent experiments immunoblots are representative of three independent experiments. Unless otherwise stated, histogram data shown represent the mean and S.D. of three independent experiments. ANOVA was used for statistical analysis. *P < 0.05, **P < 0.01. Source data are provided as a Source Data file.