Fig. 4

MGE transplantation does not alter glial responses to TBI. a Coronal brain sections 125 DAT labeled for GFAP (orange), IBA1 (magenta) and transplanted MGE cells (green). In the ipsilateral hemisphere, there was prominent glial scar surrounding the impact site. b Quantification of GFAP expression in neocortex and hippocampus in sections contralateral and ipsilateral to the injury. Ipsilateral neocortex: **P = 0.007, control vs TBI; **P = 0.009, control vs TBI-MGE; one-way ANOVA with Bonferroni post hoc test; Ipsilateral hippocampus: *P = 0.04, control vs TBI; **P = 0.008, control vs TBI-MGE; one-way ANOVA with Bonferroni post hoc test. n = 4 control mice, n = 3 TBI mice, n = 3 TBI-MGE mice. c Quantification of IBA1 immunostaining in neocortex and hippocampus in sections contralateral and ipsilateral to the injury. Ipsilateral neocortex: *P = 0.03, control vs TBI; *P = 0.03, control vs TBI-MGE; one-way ANOVA with Bonferroni post hoc test. d High resolution images of the boxed region in a labeled for GFAP (orange), IBA1 (magenta) and GFP (green). Transplanted MGE cells did not co-localize with glial markers (n = 3 mice per marker). Error bars, s.e.m.; scale bars, 500 μm (a) and 50 μm (d). Source data are provided as a Source Data file