Fig. 6
From: NAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome
Effects of NAD+ replenishment on fat metabolism in adult Day 7 wrn-1 C. elegans. We performed systematic proteomic analysis using mass spectrometry with samples from the whole-body tissues from adult D7 worms (n = 4 biologically independent experiments). We then comprehensively evaluated the changes of fat-metabolism-related molecular pathways, including mitochondrial β-oxidation, peroxisomal β-oxidation, lipolysis, glycolysis, fatty acid transport, as well as fatty acid desaturation and elongation57. a, b A summary of significantly altered proteins involved in fat metabolism are shown. Abbreviations used: ACDH: acyl-CoA dehydrogenase, ACLY: ATP citrate lyase, ACS: acyl-CoA synthetase, CPT: carnitine palmitoyl transferase, CS: citrate synthase, ECH: enoyl-CoA hydratase, FASN: fatty-acid synthase, IDH: isocitrate dehydrogenase, PFK: phospho-fructo-kinase, SCS: succinyl-CoA synthetase, SDH: succinate dehydrogenase, MDH: malate dehydrogenase. c, d Changes of fat content in the adult D7 worms treated with/without NR from L4. Oil Red staining was used for imaging with quantification using ImageJ. n = 30 worms/group (Two-way ANOVA). For c, the bars stand the independent value of mean within each group.
