Fig. 4
Identification and functional validation of the transcription factor OsNAC42 on chromosome 9. a Local Manhattan plot (top) and LD heatmap (bottom) surrounding the peak on chromosome 9. Red dashed lines indicate the candidate region for the peak. b Comparative analyses of OsNAC42 between the low and high NUE haplotypes. Boxplots for NPHR (normalized plant height ratio)-2014 (top) and NPHR-2016 (bottom) based on the haplotypes (Hap) for OsNAC42. Box edges represent the 0.25 quantile and 0.75 quantile with the median values shown by bold lines. Whiskers extend to data no more than 1.5 times the interquartile range, and remaining data are indicated by dots. Differences between the haplotypes were analyzed by Welch’s t test. c Phenotype of WT (Zhonghua11, ZH11), Tilling mutant (osnac42) and heterozygote (H), bar = 20 cm. d qRT-PCR analysis of OsNPF6.1 of WT (Zhonghua11, ZH11), Tilling mutant (osnac42) and heterozygote (H). Three biological replicates were used for qRT-PCR. e OsNPF6.1 and OsNAC42 transcript levels in different tissues. OsActin1 was used as a control, n = 3. f OsNPF6.1 and OsNAC42 expression under HN treatment from 0 to 24 h in leaf (KCl as a control of LN representing nitrogen starvation), n = 3. Data are presented as means ± SD. P values (versus the ZH11) were calculated with Student’s t test. **P < 0.01. The source data underlying Fig. 4d–f are provided as a Source Data file.
