Fig. 2

PAIso−seq captures poly(A) inclusive transcripts. a Global distribution of poly(A) tail lengths of all transcripts (CCSs) in GV oocytes. The median poly(A) tail length of CCS reads for each replicate is shown. b The distribution of poly(A) tail lengths of all genes. The median of mean poly(A) tail length of the genes for each replicate (with no less than three CCSs) is shown. c The correlation of gene (with no less than ten CCSs in each of the replicates) poly(A) tail length among three replicates of GV oocytes. The blue line represents linear regression line. The light-gray area represents confidence interval of the regression. n = 1179 (left panel), n = 1120 (middle panel), and n = 1992 (right panel). R_p and R_s refer to Pearson’s and Spearman’s correlation coefficient. d The correlation between gene poly(A) tail length in GV oocytes measured by PAIso−seq (this study) and TAIL-seq¹⁰. The blue line represents linear regression line. The light-gray area represents confidence interval of the regression. Genes with at least 10 reads in PAIso−seq and 30 tags in TAIL-seq datasets are included in the analysis (n = 1662). R_p and R_s refer to Pearson’s and Spearman’s correlation coefficient. e The distribution of poly(A) tails longer than 200 nt. The number above the bar shows the counts of CCS reads with given poly(A) tail length.