Fig. 8
From: AMPA receptors in the synapse turnover by monomer diffusion
Fast diffusing GluA1 monomers and dimers enter and exit from the synaptic region. a A typical image sequence of a fluorescent spot of Halo7-GluA1 (labeled with ATTO594; magenta spot; representative results from 13 independent video clips), entering a synaptic region (marked by Homer1b-EGFP, green spot) from the dendritic-shaft PM and exiting from the synaptic region into the dendritic-shaft PM in cultured mouse hippocampal neurons (13 DIV). Dotted lines indicate the peripheries of the dendritic shaft. b The changes of apparent D200ms for each fluorescent Halo7-GluA1 spot when it entered and/or exited from the synaptic region marked by Homer1b-EGFP (shown by small closed circles; median values are shown by larger gray open circles). The apparent D200 ms for each fluorescent spot was simply evaluated from the increment of the MSD during 200 ms, due to the limited signal-to-noise ratios for observing single molecules in the synaptic region. (Before) In the dendritic-shaft PM right before entering the synaptic region. (On Homer) During the period in the synaptic region. (After) In the dendritic-shaft PM right after exiting from the synaptic region. Lines (and closed small circles with the same color) indicate the same fluorescent spots. c The changes of the step sizes of Halo7-GluA1 spots when they entered and/or exited from the synaptic region. Bars, circles, boxes, and whiskers indicate the median values, mean values, interquartile range (25–75%), and 10–90% range, respectively
