Table 1 Summary of colocalization durations of fluorescently-labeled, tagged molecules of GluA1, GluA1ΔNTD, GluA2, and TM in the HEK293-PM

From: AMPA receptors in the synapse turnover by monomer diffusion

Molecules

Colocalizations (Oligomers)

Colocalization lifetimes (Mean ± SEM) (ms)a

P value (Log-rank test)

Probes

Temp. (°C)

Related Figures

ACP-TM (Monomer reference)

Incidental colocalization

55 ± 1*1 b

–

ATTO594

22

3, S8d

Halo7-TM (Monomer reference)

Incidental colocalization

49 ± 3*2

–

Rho110c

22

S5b, S8e

ACP-TM Halo7-TM (Monomer reference)

Incidental colocalization

52 ± 4*3

–

ATTO594 Rho110

22

4d, S9a

ACP-GluA1

GluA1 homo-Dd

164 ± 16*4,Y1

2.1 × 10−18

ATTO594

37

3d, 3e, 5a

GluA1 homo-Trid

117 ± 33*5,Y4

7.4 × 10−3

None

GluA1 homo-Td

98 ± 11*6,Y4,N5

Y41.1 × 10−3

N50.92

3e, 4e

ACP-GluA1ΔNTD

GluA1ΔNTD homo-D

100 ± 8Y4

2.1 × 10−7

ATTO594

37

3d

GluA1ΔNTD homo-T

41 ± 6Y6

8.2 × 10−10

3e

Halo7-GluA1

GluA1 homo-D

164 ± 17Y2,N4

Y24.7 × 10−9

N40.74

Rho110

37

S5b

ACP-GluA1 Halo7-GluA1

GluA1 homo-D

177 ± 11*7,Y3

1.3 × 10−25

ATTO594 Rho110

37

4d, S9b

ACP-GluA2

GluA2 homo-D

156 ± 16*8,Y1,N4

Y18.2 × 10−14

N40.60

ATTO594

37

S8d, S10a

GluA2 homo-Tri

91 ± 8*9,N5,Y8

N50.54

Y87.0 × 10−5

None

GluA2 homo-T

75 ± 9*10,N6,Y8,N9

N60.32

Y84.4 × 10−5

N90.57

4e, S8d

Halo7-GluA2

GluA2 homo-D

160 ± 18*11,Y2,N8

Y22.7 × 10−10

N80.80

Rho110

37

S8e, S8f

Halo7-GluA2 in CHO-K1 cellse

GluA2 homo-D

168 ± 9 N11

0.34

Rho110

37

S8f

ACP-GluA2 Halo7-GluA2

GluA2 homo-D

181 ± 12*12,Y3

2.9 × 10−27

ATTO594 Rho110

37

4d, S9b

ACP/Halo7-GluA1 Halo7/ACP-GluA2

GluA1/A2 hetero-Df

334 ± 34Y7,Y12

Y71.0 × 10−10

Y127.3 × 10−12

ATTO594 Rho110

37

4d

GluA1/A2 hetero-Tf

208 ± 14Y6,Y10

Y66.1 × 10−8

Y104.9 × 10−10

4e

  1. aColocalization lifetimes after correction for the photobleaching lifetimes
  2. bThe superscripts *, Y, and N indicate statistical test results. The distribution selected as the basis for the comparison is shown by the superscript *. Different numbers (1–12) indicate different bases. The superscript Y or N indicates that the distribution is or is not significantly different from that of the basis distribution, based on the p value of the log-rank test (the next column on the right) being either smaller or greater than 0.05, respectively
  3. cRhodamine110
  4. dD, Tri, and T indicate dimers, trimers, and tetramers, respectively
  5. eOnly the results shown in this line were obtained in CHO-K1 cells, for comparison with the data obtained in HEK293 cells
  6. fThe average values of the colocalization lifetimes (after correction for the photobleaching lifetimes) of heteropairs of ACP-GluA1 and Halo7-GluA2 and heteropairs of Halo7-GluA1 and ACP-GluA2 (see Supplementary Table 6)
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