Fig. 4: GUV dye-influx assay with DNA nanopores.
From: A large size-selective DNA nanopore with sensing applications
GUV experiments of opened lipidated nanopores with SRB and 40-kDa dextran–FITC (a–d) or SRB and 500-kDa dextran–FITC in outer solution (e–h). i–l GUV experiments with SRB and 40-kDa dextran–FITC in outer solution together with DNA nanopores containing 20-kDa PEG moieties immobilized in the inner channel as a size-selective plug. a, e, i Schematic of DNA nanopores penetrating lipid bilayer. b, f, j Time series of confocal images at the given intervals (hh:mm) of SRB (red), Cy5-labeled DNA nanopores (blue), and FITC (yellow). (c, g, k) Single traces of fluorescent signal from the inside of GUVs from t = 00:00 to t = 08:00 (hh:mm). d, h, l Average fluorescent traces of influx-normalized events for N-recorded events. All recorded rapid SRB influx events are included and data found in Supplementary Tables 2–4. The shaded regions surrounding the lines illustrate the standard deviations from the traces. Scale bars in (b, f, j) are 10 µm.
