Fig. 1

Identification of TCRL antibody P1C1 specific for the p53_125–134/A24 pMHC. a Binding specificity and avidity of four leads, P1C1, P1H4, P1B11 and P1A8, to a control hTERT_461–469/A24 pMHC (left) and the target p53_125–134/A24 pMHC was analysed by ELISA. b A24⁺, p53-null SaoS2 cells pulsed with 10 μM six known A24-restricted peptides were stained with 10 µg mL⁻¹ of P1C1 antibodies. Staining was observed only with cells pulsed with the p53_125–134 peptide. P1C1 binding was further analysed by c staining SaoS2 cells pulsed with a range of p53_125–134 peptide concentrations or d staining 10 μM p53_125–134 peptide-pulsed SaoS2 cells with a range of antibody concentrations. e A24⁺, mutant p53^R273H-expressing HT29 cells were stained with a range of antibody concentrations of P1C1. Data are representative of two or more independent experiments.