Fig. 1
From: m6A in mRNA coding regions promotes translation via the RNA helicase-containing YTHDC2
CDS m6A is enriched in transcripts with inactive translation. a Distribution of m6A sites along transcripts with different regional methylation. Transcripts are grouped into 5′ UTR, CDS, and 3′ UTR methylation based on the identified m6A sites. Only METTL3 or WTAP sensitive m6A sites are considered. The distribution of total m6A sites was shown in gray. UTR: untranslated region, CDS: protein coding region. b A Venn diagram shows the overlapping of transcripts containing m6A sites in different regions. c Translation efficiency (TE, left column) and m6A density across the transcriptome are presented as parallel heat-maps. Note the inverse correlation between CDS m6A methylation and TE. d Translation efficiency (TE) is plotted as accumulative fractions for mRNAs with different amount of m6A peaks in CDS. e A representative example (MAPK) of evolutionary conserved m6A sites between MEF and HEK293 cells. The coverage of m6A is shown as black lines and the input as gray. f A box plot shows the translation efficiency (TE) of mRNAs containing conserved (C) and non-conserved (NC) m6A sites in MEF and HEK293 cells (Wilcox test, all P values < 2.2 × 10−16). The median of TE in each group is indicated by a center line, the box shows the upper and lower quantiles, whiskers shows the 1.5× interquartile range, and the outliers are indicated by points. Source data are provided as a Source Data file.
