Fig. 6
ZFP161 Maintains Genomic Stability. a Generation and characterization of ZFP161 KO mice. A. Schematic representation of wild- type and gene-trapped alleles in the ZFP161 genomic locus. b PCR and Western blot analysis of genomic DNAs and lysates from ZFP161+/+, ZFP161+/−, and ZFP161−/− mice. F (exp) and F (obs) are expected and observed Mendelian frequencies, respectively, and n, the number of pups genotyped at three weeks after birth. Two-sided Fisher’s exact test, p = 0.02719. c Mice were treated with UV and ATR signaling was determined by Western blot of skin tissues. d–g Genomic stability was examined by measuring γH2AX (e), metaphase spread (f), and micronucleated normochromic erythrocytes (Mn-NCE, CD71-PI + ) (g). n = 3 independent experiments. 3 mice per genotypes, 50 metaphases per mice. h–l TCGA database analysis. Tumor mRNA expression levels were obtained from TCGA for 32 paired patient samples (h). Correlation of ZFP161 expression levels with chromosomal instability levels. Number of telomeric allelic imbalances (i), frequency of LOH (j), large scale transition (k), and weighted genome integrity index (l). m Diagram showing that ZFP161 mediates the recruitment of ATRIP/ATR to repair stalled replication fork. The graphs represent mean ± S.D., two-tailed, paired t-test. Source data are provided as a Source Data file.
