Fig. 1
From: Causative role of PDLIM2 epigenetic repression in lung cancer and therapeutic resistance
PDLIM2 is repressed in lung cancer, associating with poor prognosis. a TCGA data showing decreased PDLIM2 in human lung tumor (T, red column) compared to normal lung (NL). Sample numbers are indicated. The bottom-most and topmost horizontal lines, the lower and upper hinges, and the middle line of the boxplots indicate the minimum and maximum values, the 25th and 75th percentiles, and the median, respectively. b qPCR showing decreased PDLIM2 RNA expression in human lung tumors compared to normal lung tissues from the same patients. Percentiles of lung tumors with PDLIM2 RNA expression levels ≤0.4, between 0.4–0.8, or >0.8 of the average in normal lungs are also shown. c Lung tumor tissue microarray showing PDLIM2 repression in human lung cancer. Scale bar, 20 μm. d qPCR and immunoblotting (IB) showing decreased PDLIM2 in most human lung cancer cell lines (gray bars, n = 3). NL-20, normal human lung epithelial cell line (n = 3). e–g Kaplan–Meier survival curve generated from Kaplan–Meier Plotter showing positive association of PDLIM2 expression in tumors with overall survival (OS, e), progression free survival (PFS, f), and post progression survival (PPS, g) of lung cancer patients. h Kaplan–Meier survival curve on human lung tumor tissue microarray data in c showing positive association between PDLIM2 expression and patient survival. i qPCR and IHC staining analysis showing PDLIM2 repression in lung tumors induced by urethane in FVB/N mice (n ≥ 4). Representative lung epithelial and tumor cells are indicated by arrows. Scale bar, 10 μm. j qPCR analysis showing PDLIM2 repression in mouse lung cancer cell line MAD109 derived from a BALB/c mouse compared to normal lung tissues from BALB/c mice (n ≥ 4). k qPCR analysis showing PDLIM2 repression in mouse lung cancer cell line LLC derived from a C57BL/6 mouse, compared to normal lung tissues from C57BL/6 mice (n = 4). Student’s t test (two tailed, unpaired) was performed in (a, b, d, i–k). Data represent means ± SEM in (b, d, i–k). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
