Fig. 1
From: DNA methylation in AgRP neurons regulates voluntary exercise behavior in mice
AgRP neuron-specific knockout of Dnmt3a reduces DNA methylation in AgRP neurons. a Dnmt3a expression peaks in the postnatal ARH at P12 (n = 5–8 per timepoint). b Immunostaining for Dnmt3a shows that AgRP/NPY neurons (labeled by GFP) express Dnmt3a at P10 (inset: 63× confocal image, NPY+; DNMT3A+ neurons indicated by arrow). Scale = 10 μm. c AgRP neuron-specific Dnmt3a knockout does not affect AgRP neuronal density, t(13) = 0.55, p = 0.59, n = 5–8. d Relative to +/+ mice, AgRP neurons (labeled by SynTom) of F/F mice show reduced levels of 5-methylcytosine; left—representative immunofluorescent labeling of 5-mC in SynTom+ AgRP neurons (inset: 63× confocal image, representative AgRP neurons indicated by arrow), right—quantitation of 5-mC labeling intensity in AgRP neurons, t(8) = 2.64, p = 0.03, n = 5. Scale = 10 μm. Values reflect mean ± SEM. *p < 0.05. Source data for a, c, d are provided as a Source Data file.
