Fig. 1
From: Structural basis of denuded glycan recognition by SPOR domains in bacterial cell division
Cell envelope constriction in Gram-negative bacteria. a Components of PG in Gram-negative bacteria and production of denuded glycan in septal PG by amidases. NAM is highlighted in dark green, NAG in light green and 1,6-anhydroNAM (anhNAM) in red. b Top, scanning-electron micrograph showing advanced cytokinesis between P. aeruginosa PAO1 daughter cells. Down, schematic cartoon adapted from Gray et al.30 representing the cell-division septum and coordinated processes involved in cell-envelope constriction. c Close-up diagram of the division site (corresponding to the black boxed area in (b), according to the model first put forth by Jorgenson et al.23, showing the coordinated action of peptidoglycan amidases (purple) and the glycolytic activity of RlpA upon recognition of the naked glycan chains by its SPOR domain (in yellow surface). OM outer membrane, IM inner membrane. The catalytic DPBB domain of RlpA is colored in gray. The protein has a covalently attached lipid at the amino terminus.
