Fig. 4

Pre-pubertal Lgr5⁺ populations are stem/progenitor cells for the developing uterus. a–e Endogenous tdTom fluorescence in Lgr5-2A-CreERT2; R26-tdTomato mouse uterus traced from P7 to P8 a, P7–P14 b, P7–P56 c, P14–P16 d, and P14–P56 e, respectively. Insets with solid and dashed lines indicate developing glandular epithelium (GE) and luminal epithelium (LE), respectively. White arrows indicate differentiated GE. St Stroma. f, g Co-IF for tdTom and Foxa2 in Lgr5-2A-CreERT2; R26-tdTomato mouse traced from P7 to P56 f and P14–P56 g. h Comparison of the percentage of Fox2⁺ cells within tdTom⁺ tracing units in P7–P56 and P14–P56 traced mice. Three independent fields from each mouse were analyzed. Data are represented as mean ± s.e.m. of the average of five independent mice. Data were tested for significance using unpaired two-tailed t-test (P = 9E−05, ***P < 0.001). i 3D image of P14 Lgr5-2A-EGFP mouse uterus, co-stained for Epcam and Foxa2. j, k Co-IF for tdTom and K8 in Lgr5-2A-CreERT2; R26-tdTomato mouse traced from P7 to P56 j and P14–P56 k. Insets with solid and dashed lines indicate mature GE and LE, respectively. Scale bars, 100 μm.