Fig. 1
AAG associates with active transcription and regulates gene expression. a Immunoblot analysis of chromatin fractionation assay indicating AAG distribution in total fraction (TF), soluble supernatant fraction (SF) and chromatin fraction (CF). Histone H3 and α-tubulin served as controls. b Quantification of three independent experiments as the one depicted in a; error bars represent mean ± SEM (n = 3). c Immunoprecipitation of AAG from HEK293T whole-cell extracts (WCEs) untreated or treated with DNaseI, Mnase, and RNaseI, showing the interaction with RNA polymerase II phosphorylated at Serine 2 (S2P) of CTD repeat. d Immunoblot analysis of WCEs from HEK293T WT and AAG−/− cells generated by CRISPR-Cas9 technology. e Heat map of the expression levels of AAG-regulated genes in HEK293T cells. Color scale is representing change in the gene expression depicting log2 fold change relative to the mean. f Top six biological processes (BP) gene ontology (GO) terms as determined by the Database for Annotation, Visualization and Integrated Discovery (DAVID) for genes dysregulated in HEK293T AAG−/− cells when compared to WT. g Up- and down-regulated differentially expressed genes (DEGs) in HEK293T AAG−/− cells. h, i Top six BP GO terms as determined by DAVID for genes upregulated (h) and downregulated (i) genes. Source data are provided as Source Data file.
