Fig. 3

Elongator and AAG co-regulate gene expression. a Immunoblot of HEK293T whole-cell extracts (WCEs) from WT, AAG^−/−, ELP1^−/−, and AAG^−/−ELP1^−/− cell lines generated via CRISPR-Cas9 technology. b Top six biological processes (BP) gene ontology (GO) terms as determined by DAVID for genes dysregulated in HEK293T ELP1^−/− cells when compared to WT cells. c Downregulated and upregulated differentially expressed genes (DEGs) in HEK293T ELP1^−/− cells. d Venn diagrams of AAG- and ELP1-regulated genes in HEK293T cells. e Top six BP GO terms as determined by DAVID of genes co-regulated by AAG and ELP1 in HEK293T cells. f Directionality of DEGs regulated by AAG and ELP1 in HEK293T cells. g–j Relative mRNA levels of ALDH1A2 (g), CRMP1 (h), CDH23 (i) and YTHDC1 (negative control) (j) genes in HEK293T WT, AAG^−/−, ELP1^−/− and AAG^−/−ELP1^−/− cells. Error bars represent mean ± SEM (n ≥ 3). **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001, one-way ANOVA. Source data are provided as Source Data file.