Fig. 4
From: Tsc1-mTORC1 signaling controls striatal dopamine release and cognitive flexibility
Loss of Tsc1 reduces striatal DA release probability. a Mean ± SEM dorsolateral striatal [DA]o versus time evoked by single-pulse stimulations (1p) delivered 3 s apart in 5 mM extracellular Ca2+ (1 μM DHβE applied throughout). Traces are an average of 16 transients per genotype from four mice. b Mean ± SEM ratio of peak [DA]o in the dorsolateral striatum evoked by the second-to-first pulse in 5 mM extracellular Ca2+ (1 μM DHβE applied throughout). **p = 0.0055, paired two-tailed t test. c Mean ± SEM [DA]o versus time evoked by single-pulse (1p) or high-frequency stimulation (four pulses at 100 Hz, 4p) in the dorsolateral striatum in aCSF. Traces are an average of 18 transients for 1p and nine transients for 4p from five mice per genotype. d Mean ± SEM ratio of peak [DA]o evoked by a four-pulse train to single-pulse stimulation in the dorsolateral striatum in aCSF (n = 5 mice per genotype), 1 μM DHβE (n = 5 mice per genotype), or 5 mM extracellular Ca2+ (n = 4 mice per genotype). *p = 0.0204; **p = 0.0024; ***p = 0.0009, paired two-tailed t tests. For all bar graphs, dots represent values for individual mice. See also Supplementary Fig. 2. Source data are provided as a Source Data file.
