Fig. 1: GCase activity is reduced in DA neurons with LRRK2 mutations. | Nature Communications

Fig. 1: GCase activity is reduced in DA neurons with LRRK2 mutations.

From: LRRK2 kinase activity regulates lysosomal glucocerebrosidase in neurons derived from Parkinson’s disease patients

Fig. 1

Live-cell measurement of fluorescent unquenching resulting from hydrolysis of the artificial GCase substrate PFB-FDGlu by lysosomal GCase in LRRK2 G2019S (a left panel) and R1441G/C (b left panel) DA neurons relative to healthy controls over 90 min. GCase activity was determined by analyzing the relative slope of these measurements (a, b right panel). Sanger sequencing results from LRRK2 G2019S c and R1441C d lines  and subsequent isogenic controls generated using CRISPR/Cas9. Relative lysosomal GCase activity in DA neurons with LRRK2 G2019S e and R1441C f mutations compared to isogenic corrected controls. The data are presented as the mean ± SEM, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001, using one-way ANOVA followed by Tukey’s multiple comparison post hoc test a, b, or paired two-tailed t-test e, f. Source data are provided as a Source Data file.

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