Fig. 6: Overexpression of Rab10 WT increases GCase activity in fibroblasts and DA neurons. | Nature Communications

Fig. 6: Overexpression of Rab10 WT increases GCase activity in fibroblasts and DA neurons.

From: LRRK2 kinase activity regulates lysosomal glucocerebrosidase in neurons derived from Parkinson’s disease patients

Fig. 6

Relative GCase activity in control fibroblasts transduced with lentivirus expressing GFP, Rab10 WT or Rab10 T72E a–c. Representative western blots showing overexpression of Rab10 and Rab10 T72E in control (CTRL) fibroblasts (a right). Relative GCase activity in DA neurons containing LRRK2 R1441C d, LRRK2 G2019S e, GBA1 N370S f, or GBA1 E326K g mutations transduced with CTRL or Rab10 lentivirus for 10 days, with representative western blots showing overexpression of Rab10 in DA neurons (f right). The data are presented as the mean ± SEM, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001, using one-way ANOVA followed by Tukey’s multiple comparison post hoc test a–c, or paired two-tailed t-test d–g. Source data are provided as a Source Data file.

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