Fig. 3: Molecular and functional assessment of immature B subpopulations.
From: Classification of mouse B cell types using surfaceome proteotype maps
a The immature B population was further divided into three subpopulations defined by CD20 and CD180 fluorescence intensity: double negative (DN), positive for CD20 (SP), and positive for both (DP). b In-vitro differentiation of the defined immature B subpopulations during three days of culturing. c Fraction of IgD+ cells in immature B subpopulations. d Mean fluorescence intensity for BAFF receptor for the three immature B subpopulations. e Modulation of in vitro survival of subpopulations with BAFF treatment. f Principal component analysis of proteotype data reflects the identified immature B subpopulations. Error bars indicate standard deviation. Statistical analysis was done with two-tailed unpaired Student’s t test. One star indicates P < 0.01; four stars indicate P < 0.0001.
