Fig. 6
From: CD4+ T cell help creates memory CD8+ T cells with innate and help-independent recall capacities
Help delivered during priming leaves an epigenetic imprint in steady-state memory cells. Mice (n = 3 per group) received Help or No Help vaccine on days 0, 3, and 6. At day 100, E7-specific memory CD8+ T cells were isolated from the spleens and subjected to ChIPseq analysis with antibodies against H3K4me3 and H3K27me3. a PCA plots depicting results of ChIPseq analysis. b, c Volcano plots depicting differentially modified regions (DMRs) as designated by H3K4me3 (b) or H3K27me3 (c) marks. Blue and red dots indicate DMRs with significant differential modifications (FDR < 0.05). d Molecules differentially modified by H3K4me3 in helped memory E7-specific CD8+ T cells, as assigned to functional categories and annotated for subcellular localization by IPA. e, f Correlation between differential mRNA expression in helped versus non-helped TEM (e) and TCM (f) cells and histone methylation in gene bodies and regions maximally 1 kb upstream from the TSS. Normalized tags of H3K4me3 and H3K27me3 are shown versus average normalized mRNA expression values in helped versus non-helped TEM (e) and TCM (f) cells (described in Fig. 5).
