Fig. 2: Mass cytometry time-series analysis offers deep single-cell resolution of canonical EMT states.

a Workflow schematic of cell culture conditions for TGFβ time-course (addition and withdrawal (W) time-points) and mass cytometry analysis. Arrows indicate times at which cells were collected for both analysis and re-seeding for the remaining time-points (see also Supplementary Table 1, Figs. 2 and 3, and Methods). b Representative images of HCC827 cells at each time-point (magnification ×10, scale bar 200 μm) with respective E-Cadherin/Vimentin and CD44/CD24 mass cytometry plots shown below. Arrows indicate the previously observed canonical EMT marker changes and states, respectively. c Histogram overlays illustrating EMT marker expression distributions in HCC827 cells undergoing EMT and MET. On the right, a heatmap summary of the canonical EMT marker fold (arsinh) changes relative to state E when analyzed in the four EMT states individually at the 10-day TGFβ time-point. d Additional marker distributions in HCC827 cells undergoing EMT and MET (see Supplementary Fig. 3 for remaining markers and an independent biological replicate experiment).