Fig. 5: EMT–MET PHENOSTAMP serves as a means to assess EMT–MET states in independent NSCLC cell lines. | Nature Communications

Fig. 5: EMT–MET PHENOSTAMP serves as a means to assess EMT–MET states in independent NSCLC cell lines.

From: Mapping lung cancer epithelial-mesenchymal transition states and trajectories with single-cell resolution

Fig. 5

a Projection of NSCLC cell lines onto the EMT–MET PHENOSTAMP (top) and their respective morphological assessment (×10 images, scale bar 200 μm, bottom). HCC827 samples (ctrl, 2-day TGFβ) were ran on a different day than the ones used for the map construction for validation (left). A549 and H3255 cells were treated with TGFβ for 10 and 13 days, respectively, and analyzed with mass cytometry. b Shown are the respective to each cell line and condition expression profiles of E-Cadherin (top) and Vimentin (bottom) visualized on the EMT–MET PHENOSTAMP. c E-Cadherin/Vimentin and CD44/CD24 mass cytometry plots per projected cell line sample. See Methods and Supplementary Fig. 8 for further details.

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