Fig. 4

Sesn1-3 triple knockout mice display drastic impairment in exercise response. 5-month-old WT and Sesn1^−/−/Sesn2^−/−/Sesn3^−/− (TKO) mice (C57BL/6 background) were subjected to voluntary wheel running for 1 month. Running distance (a), speed (b) and time (c) were recorded. Mice were then subjected to incremental exercise (forced running of increasing intensity until exhaustion) with indirect calorimetry (d–l). VO₂, oxygen consumption rate. VO₂ basal, baseline VO₂. VO₂ max, maximum VO₂. ΔVO₂, VO₂ max − VO₂ basal. VCO₂, CO₂ production rate. RER, respiratory exchange ratio. RER basal, baseline RER. RER max, maximum RER. ΔRER, RER max – RER basal. Biologically independent animals: n = 5, 4 (a–l; WT and TKO mice, respectively). Error bars, s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001 or ns, non-significant from a two-tailed student’s t test.