Fig. 1

1H-15N-HSQC spectra of HBP-1 at different pH values. a HBP-1 in the initial solution state at pH 3.3. b Dilute phase after LLPS at pH 6.5 (after sedimentation of coacervate microdroplets). c Overlay of the two spectra. Spectra acquired at 298 K and a protein concentration of 2 mg mL−1 (130 μM).