Fig. 1
Detecting de novo mutations (DNMs) in the Cas9-treated monkeys. a The analyzed trios of rhesus monkeys (rm) and cynomolgus monkey (cm). F father, M mother, O offspring, ko knockout, ki knockin, B brain, L liver, and M muscle. b The pipeline of variant calling, filtering, and DNMs identification. HC.DNM-high confident DNMs. c Venn diagram of the high-confident DNMs for rmO2-Bko identified by overlapping candidate DNMs from four different calling tools. d Summary of the identified high-confident DNMs. The circles refer to Ca9-treated rhesus monkeys and the triangle refers to the Ca9-treated cynomolgus monkey. The green shadow indicates the range of expected DNMs (22–78) per generation given the known spontaneous mutation rates of primates.
