Table 1 Summary of trio WGS and identification of DNMs.

rmF^wt

Father

Wildtype

45.81

–

–

–

rmM1^wt

Mother-1

Wildtype

46.23

–

–

–

rmM2^wt

Mother-2

Wildtype

45.23

–

–

–

rmO1^ko

Offspring-1

Cas9-treat

48.39

0.435

1324

34

5

rmO2-B^ko

Offspring-2-brain

Cas9-treat

45.98

0.953

1169

29

3

rmO2-M^ko

Offspring-2-muscle

Cas9-treat

44.85

0.938

1188

30

3

rmO2-L^ko

Offspring-2-liver

Cas9-treat

45.35

0.952

1,230

29

2

rmO3^ko

Offspring-3

Cas9-treat

46.84

0.122

1267

38

1

rmO4^ko*

Offspring-4

Cas9-treat

45.92

0

1531

30

1

rmO5^ko

Offspring-5

Cas9-treat

47.60

0.321

1847

37

1

cmF^wt

Father

Wildtype

64.08

–

–

–

cmM^wt

Mother

Wildtype

76.11

–

–

–

cmO^ki

Offspring

Cas9-treat

68.42

–

6060

32

3