Fig. 6
Gene modules correlated with forming nuptial spines in Leptobrachium leishanense. a Correlation between coexpressed modules and sampling traits. Heatmap colors represent correlation coefficients (*Fisher’s asymptotic p < 0.05; **p < 0.01). Rows are different modules, and columns are different sampling traits. b An illustration of the canonical Wnt pathway. Genes marked with red squares are included in the ME03 module; the gene marked with a red star is under significant positive selection in L. leishanense compared to other anurans. c Weighted coexpression network for genes in the ME05 module. Dark-blue circles are keratin genes; yellow circles are genes encoding protein-glutamine gamma-glutamyltransferase 2 (tgl2); the black circle is tyr gene that is annotated as a melanin synthesis-related gene; the black diamond is a transcriptional factor (hoxc13); light-blue circles are other annotated genes involved in diverse metabolic processes; gray circles are unknown genes. Colored lines represent the weight of connectivity between genes, with white to red indicating weights from low (0.10) to high (0.78). The node sizes are associated with the kME values ranging from 0.71 to 1.00. stard10 StAR-related lipid transfer (START) domain containing 10, cat catalase, tgl2 protein-glutamine gamma-glutamyltransferase 2, cyp2m1 cytochrome P450 2M1, hoxc13 homeobox protein Hox-C13, sspo SCO-spondin (precursor), slc16a9 monocarboxylate transporter 9, unc93a Protein unc-93 homolog, tyr tyrosinase, pcol protein piccolo-like, bmper BMP-binding endothelial regulator, prss3 trypsin-3.
