Fig. 1

Overexpression of LRIG1 in PCa and correlation with better patient survival. a–c Elevated LRIG1 mRNA in PCa in Oncomine (a, b) and in TCGA (c). In b, the LRIG1 mRNA levels were extracted from tumors (T) in the 15 Oncomine datasets (a) and presented as fold changes (FC) after normalizing to the corresponding normal/benign tissues (N). The error bar represents the mean±S.D. In c, the center lines represent median values, box edges are 75th and 25th percentiles, and whiskers represent the maximum and minimum values, respectively. d High LRIG1 mRNA levels correlate with better patient overall survival (the Setlur dataset). P-value was determined using the Log-Rank test. e, f Prostate tumors overexpress LRIG1 protein. Shown in e is LRIG1 IHC (using Sigma mAb, 1:100 dilution) in 2 HPCa (i.e., HPCa90 and HPCa139) and matched benign tissues. Enlarged images of boxed regions are shown on the right. Scale bars are indicated in one representative whole-mount (left) and enlarged (right) image, respectively. In f, whole-mount slides were scanned by Aperio Scanscope and used to quantify LRIG1 expression levels. The centerline represents the median, box edges are 75th and 25th percentiles, and whiskers represent maximum and minimum values. g Increased LRIG1 protein expression in PCa. LRIG1 staining intensity was determined in 3 TMAs that contained both tumors (T) and normal (N) tissues (total numbers indicated below). The centerline represents the median, box edges are 75th and 25th percentiles, and whiskers represent 95% and 5% values, respectively. The P-values in b, c, f, and g were determined by two-tailed unpaired Student’s t-test. *Source data for Fig. 1f, g are provided as a Source Data file.